Media for E. coli fed-batch fermentation?

I work for a small CRO/consulting company and I would like to implement a few different E. coli fed-batch fermentation processes to document to clients what can be achieved by fed-batch fermentation compared to batch with regards to biomass and product formation. So far I have been using a minerals medium process by Korz et al. (1995) with some success and I have been running another process with huge amounts of yeast extract in both batch medium and feed, but that one tends to foam enourmously after a few days. What do you have positive experience with?

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  • 3 weeks ago

    Protein expression in E.coli & various systems is offered in Leading Biology

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