Anonymous
Anonymous asked in Science & MathematicsBiology · 4 weeks ago

An EMSA can be used to determine if a transcription factor is bound to DNA.?

true or false. 

Is it true since EMSA is somewhat similar to elisa where they both need to be bounded to a DNA

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  • Ted K
    Lv 7
    4 weeks ago

    True.  But it's not similar to ELISA at all.  The respective principles behind ELISA versus EMSA assays are completely different.

  • 4 weeks ago

    EMSA can be used qualitatively to identify sequence-specific, DNA-binding proteins (such as transcription factors) in crude lysates and, in conjunction with mutagenesis, to identify the important binding sequences within the upstream regulatory region of a given gene.

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