An EMSA can be used to determine if a transcription factor is bound to DNA.?
true or false.
Is it true since EMSA is somewhat similar to elisa where they both need to be bounded to a DNA
- Ted KLv 74 weeks ago
True. But it's not similar to ELISA at all. The respective principles behind ELISA versus EMSA assays are completely different.
- 4 weeks ago
EMSA can be used qualitatively to identify sequence-specific, DNA-binding proteins (such as transcription factors) in crude lysates and, in conjunction with mutagenesis, to identify the important binding sequences within the upstream regulatory region of a given gene.