This  from Japan. The situation overseas is unknown. COVID-19Are those people asymptomatic? Have you had a PCR test? Is it an antibody test?

This is a message from Japan. I am a specialist in molecular biology and immunology. The situation abroad is unknown. Are there any survivors? We are told that there are 50,000 new cases of infection in the United States every day. Are those people asymptomatic? How bad is it?

Have they had a PCR test? Is it an antibody test?

I felt that there was no uniformity in the content of the inspection in each country.

If you have expertise in molecular biology and immunology, please tell me the information on the viral genomes that are being tested for in the United States.

For East Asia, the National Institute of Infectious Diseases in Japan has cultured and distributed them. We have those resources, but they do not take into account the mutants. It doesn't take into account protein modification. In other words, no one knows exactly what it is.

Most countries, including Japan, do very little PCR testing, either. No antibody or antigen testing has been done. We have no money. We believe the same is true for Southeast Asia, China, Russia, etc., and we do very little testing. I thought the same was true for the US. In other words, we believe the true number of people infected is thousands of times higher than what has been published. Is that correct?

1 Answer

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  • 1 month ago

    PCR suffers with drawbacks that limit it to diagnosis during the acute phase of infection and cannot meet the time sensitive needs. To better understand the transmission dynamics of SARS-CoV-2 and develop effective countermeasures against it, antibody-based immunoassays will be essential.

    As 2019-nCoV and SARS N proteins have 90% homologous similarities, it is allowed that antibodies and antigens to SARS N protein to be used for diagnostic testing for 2019-nCoV. Diagnostic testing for the novel Coronavirus 2019-nCoV is undertaken with 2 main approaches: genome sequencing and real-time reverse transcriptase PCR. Sequencing was used primarily in the early days of the outbreak for initial identification of this novel virus and is largely a tool of viral discovery. 

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