Hello this is probably really really easy. That's the question. I jut put two arrows on either side pointing at each other for the reverse and forward primers, In not sure if its just as simple 2^n finding the quantity of DNA since it was run for 25 cycles. However that doesn't make sense because it asks for the predicted size not the quantity/amount of DNA. Also if by chance anyone would know what to do for 9 EXCELLENT. for that question we were supposed isolate bacterial colonies from environmental sources, and then PCR them later on. the only reason i can possibly think that it would be important that it be bacteria and not fungi is that bacteria are easier to identify ( gram neg vs gram pos, morphology,)
Any help greatly appreciated
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